24-hour detection of enterococci

Drinking Water Bottled Water Recreational Water Wastewater Groundwater

Detects enterococci in a 100 mL format. Results in 24 hours. 


The Enterolert® Test gives you accurate results overnight so you can act quickly to protect the public and public resources:

  • Discover enterococci contamination in half the time of standard methods.
  • Reopen beaches in just 24 hours.
  • Eliminate tedious membrane filtration work.


Enterolert is widely approved and accepted:


Which IDEXX enterococci test is right for you? View a comparison.  



Call 1-800-321-0207 or email water@idexx.com.

Enterolert: Be confident in your outcomes




  • No media preparation.
  • No autoclaving.
  • No colony counting.
  • No glassware cleaning.


  • Less than 1 minute of hands-on time.
  • Results in 24 hours rather than 48 to 72 hours.




  • Sensitive to 1 enterococci/100 mL.
  • Enumerates up to 2,419 enterococci per 100 mL without dilutions (with Quanti-Tray®/2000).
  • Less subjective interpretation.
  • 50% fewer false positives and 95% fewer false negatives than the standard membrane filtration (MF) method.1


  • Up to 12-month shelf life minimizes waste.
  • 24-hour test saves incubator space.


Budnick GE, Howard RT, Mayo DR. Evaluation of Enterolert for Enumeration of Enterococci in Recreational Water. Appl Environ Microbiol. 1996;62:3881–3884.
Resource List
Product Information

Enterolert® (20-test pack)
Catalog Number: WENT020

Enterolert® (200-test pack)
Catalog Number: WENT200


Combo packs:

Snap packs and Quanti-Trays together.

How Enterolert® works


Enterolert uses a Defined Substrate Technology® (DST®) nutrient indicator to detect enterococci. This nutrient indicator fluoresces when metabolized by enterococci. DST improves accuracy and avoids the need for hazardous sodium azide suppressants used in traditional media.



View/Print Enterolert Procedure 

Learn how to use Enterolert




Learn how to use Quanti-Tray




Learn how to use Quanti-Tray/2000






A step-by-step guide to using Enterolert


Step 1

Add reagent to sample.


Step 2

Pour into Quanti-Tray® (counts from 1–200) or Quanti-Tray®/2000 (counts from 1–2,419).


Step 3

Seal using Quanti-Tray® Sealer and incubate for 24 hours at 41°C ±.5°C.


Step 4

Count fluorescent wells and refer to most probable number (MPN) table.


(counts from 1–200)



(counts from 1 to 2,419)


Frequently asked questions and answers


Is Enterolert U.S. EPA-approved?

Yes, Enterolert is U.S. Environmental Protection Agency (EPA)-approved as a method for enterococci detection in following types of water:

  • Ambient waters—includes fresh, marine or estuarine surface water used for recreation, propagation of fish, shellfish, or wildlife; agriculture; industry; navigation; or as source water for drinking water facilities
  • Ground water
  • Wastewater


What species of enterococci can Enterolert detect?

Some species of enterococci that Enterolert detects are: faecalis, faecium, avium, gallinarum, casseliflavis, and durans.


What is the detection limit of Enterolert?

Enterolert detects enterococci at 1 organism/100 mL.


What is the shelf life of Enterolert?

Enterolert shelf life is up to 12 months from the date of manufacture.


What are the recommended storage conditions for Enterolert?

Store at 2–30°C away from light.


What color and consistency should the Enterolert powder be?

The Enterolert reagent powder should be tan in color; it should be dry and free-flowing. Any concerns about the color or integrity of the powder should be directed to IDEXX Technical Service at +1-800-321-0207 or by email at water@idexx.com.


Can I quantify my Enterolert results?

Yes, Enterolert can be run with Quanti-Tray®, Quanti-Tray®/2000 or any multiple-tube format.


Is there a comparator for Enterolert?

There is no comparator for Enterolert. For comparison, use a negative control when interpreting results.


What are the disposal requirements for an Enterolert sample?

Check with your local, state and/or federal authorities for proper disposal of bacteriological biohazard materials at your facility.


Enterolert: Everything you need for confident outcomes


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