Enterolert

24-hour detection of enterococci


               

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Overview

Easy
  • No media preparation.
  • No autoclaving.
  • No colony counting.
  • No glassware cleaning.

Rapid 
  • Less than 1 minute of hands-on time.
  • Results in 24 hours rather than 48 to 72 hours.
Accurate
  • Sensitive to 1 enterococci per 100 mL.
  • Enumerates up to 2,419 enterococci per 100 mL without dilutions (with Quanti-Tray/2000).
  • Less subjective interpretation.
  • 50% fewer false positives and 95% fewer false negatives than the standard membrane filtration (MF) method.1
Economical
  • Up to 12-month shelf life minimizes waste.
  • 24-hour test saves incubator space.

Science

How the Enterolert Test works

The Enterolert Test uses a proprietary Defined Substrate Technology (DST) nutrient indicator to detect enterococci. This nutrient indicator fluoresces when metabolized by enterococci. DST improves accuracy and avoids the need for hazardous sodium azide suppressants used in traditional media.

How to use

 

Learn how to use the Enterolert Test


Quantification


Step 1

Add reagent to sample.

Step 2

Pour into Quanti-Tray (counts from 1–200) or Quanti-Tray/2000 (counts from 1–2,419).

Step 3

Seal using Quanti-Tray Sealer and incubate for 24 hours at 41°C ± 0.5°C.

Step 4: Quanti-Tray

For counts from 1–200: Count fluorescent wells and refer to most probable number (MPN) table.

Step 4: Quanti-Tray/2000

For counts from 1–2,419: Count fluorescent wells and refer to most probable number (MPN) table.

Frequently asked questions

 

Yes, the Enterolert Test is U.S. Environmental Protection Agency (EPA)-approved as a method for enterococci detection in following types of water:

  • Ambient waters—includes fresh, marine, or estuarine surface water used for recreation, propagation of fish, shellfish, or wildlife; agriculture; industry; navigation; or as source water for drinking water facilities
  • Ground water
  • Wastewater

Some species of enterococci that Enterolert Test detects are: faecalis, faecium, avium, gallinarum, casseliflavis, and durans.

Enterolert Test detects enterococci at 1 organism/100 mL.

The shelf life of the Enterolert Test is up to 12 months from the date of manufacture.

The Enterolert reagent powder should be tan in color; it should be dry and free-flowing. Any concerns about the color or integrity of the powder should be directed to IDEXX Technical Services.

Yes, the Enterolert Test can be run with Quanti-Tray, Quanti-Tray/2000 or any multiple-tube format.

There is no comparator for the Enterolert Test. For comparison, use a negative control when interpreting results.

Check with your local, state, and/or federal authorities for proper disposal of bacteriological biohazard materials at your facility.

Resources & Tools

Tests & Accessories

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Resources

IDEXX Water has reference materials and approval documents to support the many products in our water portfolio. Find the document(s) you need by selecting the link below.

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Product Information

Enterolert Test (20-test pack)
Product Number: 98-21374-00

Catalog Number: WENT020

Enterolert Test (200-test pack)
Product Number: 98-21375-00

Catalog Number: WENT200

Snap pack and Quanti-Tray bundles are available.

Water Customer Support

Corporate Headquarters
IDEXX Laboratories, Inc.
One IDEXX Drive
Westbrook, Maine 04092 USA

Tel: 1-800-321-0207
Fax: 1-207-556-4630

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ISO Certifications

Locations and ISO Certificates

References

1.  Budnick GE, Howard RT, Mayo DR. Evaluation of Enterolert for Enumeration of Enterococci in Recreational Water. Appl Environ Microbiol. 1996;62:3881–3884.