Enterolert-DW^*

The Enterolert-DW Test is designed to detect Enterococcusspecies in drinking water samples. It is based on IDEXX’s proprietary Defined Substrate Technology (DST). The Enterolert-DW Test utilizes ortho-nitrophenyl-ß-D-glucoside as a nutrient indicator and incorporates a specifically designed blue background color in its formulation. When the enzyme substrate is metabolized by the enterococci, the sample turns from blue to green to indicate a positive detection. Any change from the original color to green is considered a positive result. No ultraviolet light source is required.

The Enterolert-DW Test is designed to detect enterococci in drinking water. As with any microbial method, some Enterococcus species are more easily detected than others. Some examples of Enterococcus species we have detected in trials and studies are:

Enterococcus faecalis
Enterococcus faecium
Enterococcus hirae
Enterococcus gallinarum
Enterococcus casseliflavus
Enterococcus durans
Enterococcus avium

There is no comparator for the Enterolert-DW Test. For comparison, use a negative control when interpreting results.

The Enterolert-DW Test is incubated at 41°C ± 0.5°C for 24 hours and the results are valid up to 28 hours, unless your local regulations indicate differently.

Yes, if they are positive. An inoculated Enterolert-DW Test sample that is positive for enterococci before 24 hours is a confirmed positive test for enterococci.

Yes, if they are negative. If an inoculated Enterolert-DW Test sample is inadvertently incubated over 28 hours, lack of green color is a valid negative test; a green color after the 28 hours is not valid and should be repeated or verified.

Yes, both the Quanti-Tray and Quanti-Tray/2000 can be used with the Enterolert-DW Test.

Because the Enterolert-DW Test is used with the Quanti-Tray system, any sterile vessel can be used.

Store the Enterolert-DW Test at 2–25°C, away from direct light and humidity.

When the enzyme substrate is metabolized by the enterococci, the sample turns from blue to green to indicate a positive result. Any change from the original color to green is considered a positive result.

The Enterolert-DW Test is designed specifically for drinking water testing. Extensive studies were performed on enterococci stressed by chlorine, to mimic the impact of water treatment.

The Enterolert-DW Test may be used for other water types. Before implementing testing, IDEXX recommends you use a typical laboratory validation protocol to assure that any other water types will work with this product before implementation.

The Enterolert-DW reagent powder should be white to off-white in color with a slight greenish tint; it should be dry and free-flowing. Any concerns about the color or integrity of the reagent powder should be directed to IDEXX Technical Support.