With the Filta-Max xpress system you can process samples in two minutes or less with the push of a button.
The Filta-Max xpress system offers a variety of sample collections (PDF) and processing choices. Simply choose the option that works best for you.
The Filta-Max xpress system has utilized IDEXX expertise in foam filtration technology to create a breakthrough in Cryptosporidium and Giardia capture and recovery.
While retaining all the benefits of foam technology, the new filter has been specifically designed to:
Filter module: The filter module is constructed of 40 reticulated foam discs with a diameter of 5.5 cm (with an 1.8 cm diameter hole), and 39 discs with a diameter of 4.0 cm (also with an 1.8 cm diameter hole). All the discs are 1 cm in depth.
Discs: The discs are stacked alternately to a height of 79 cm and then compressed to a height of 3.0 cm. A retaining bolt maintains the compression. This produces a filter module consisting of an outer region with a 13.3X compression ratio and an inner core with a compression ratio of 26.6X.
Filter housing: The filter is placed in a reusable filter housing for sampling.
Sampling process: During the sampling process, the outer region acts as a prefilter and the inner core as a barrier. As a result, the majority of target organisms become trapped at the interface between the inner and outer regions, with larger particulates caught in the prefilter.
The Filta-Max xpress system uses air pressure to force a specifically designed elution buffer through the filter.
The action of the buffer, along with the shear forces generated inside the filter, result in the fast, efficient elution of target organisms from the filter matrix.
Watch these videos to learn how to do several operations using the Filta-Max xpress.
Elution using Filta-Max xpress
Place the Filta-Max xpress filter module in the filter housing and connect it to the required source.
(IDEXX supplies quick-connect fittings to enable easy field or grab sample handling.)
After sampling, the filter housing can be attached directly to the IDEXX Filta-Max xpress Pressure Elution Station. (Other sampling options are available.)
Is the Filta-Max xpress system accepted by the U.S. EPA?
Yes, the Filta-Max xpress system is accepted by the U.S. Environmental Protection Agency (EPA) under Methods 1622 and 1623.
What volume remains in the housing after sampling?
Typically there might be between 0 mL–50 mL remaining in the housing after sampling. This is purged from the housing during the first cycle of the elution.
Can I use my existing Filta-Max MKI housing and my housing with hosetail fittings with the Filta-Max xpress system Pressure Elution Station?
You can use only Filta-Max housings (MKII) with the correct fittings with the Pressure Elution Station. You can use Filta-Max MKI housings and Filta-Max MKII housings with hosetail fittings for field sampling; however, you should place the filter modules in an MKII housing with appropriate fittings for the elution process.
Can I convert my MKII housings for use with the Filta-Max xpress Pressure Elution Station?
Yes. IDEXX provides a kit that includes a QF stem and a QC body that you can fit onto the MKII housing. IDEXX also provides a sampling kit that you can attach to the sampling hose to aid in the quick attachment of the filter housing.
Which pressures do you recommend for use with the Filta-Max xpress Pressure Elution Station?
Can you suggest transport containers for the Filta-Max xpress filter module after sampling?
The Qorpak plastic 8 oz jar can be used to transport the filters. VWR cat # 36318-860. The Qorpak cat # is PLC-4DK0182AWNA6C9 3823.
Can I purchase the Swagelok fittings elsewhere?
Yes, they can be purchased directly from Swagelok.
Qorpak is a registered trademark of All-Pak, LLC. Swagelok is a registered trademark of the Swagelok Corporation.
PRESSURE ELUTION STATION QUESTIONS
What is the final volume after elution?
The final volume is 400 mL–450 mL.
Are there any integrity effects on the oocysts and cysts?
IDEXX performed experiments with various ages of oocysts and cysts, and did not see any changes to the morphology or mophometry.
Do you see more consistent or higher recoveries with the Filta-Max xpress system?
Recoveries are equivalent to, or higher than, Filta-Max and show a higher degree of precision.
Can I split the sample in two after elution?
Yes, this is possible.
What is in the elution buffer used in the Filta-Max xpress Pressure Elution Station?
The Filta-Max xpress buffer is Phospate Buffered Saline with Tween 20 (PBST). You can find directions on how to prepare this PBST elution buffer on page 5 of the Filta-Max xpress Operators Guide (Biological) .
Does the lid on the buffer carboy fit onto a larger volume carboy?
The lid is interchangeable with a larger carboy with an 83-mm diameter closure.
How should I clean the pressure chamber after each run?
You should run reagent water through the elution station after the last elution cycle of the day.
How should I clean the QC body on the Pressure Elution Station?
You can clean the QC stem with an alcohol wipe; however, positive pressure is used to elute so there is very minimal risk of cross-contamination.
When centrifuging a larger volume, such as 400–450 mL, is there anything I should take into consideration to maximize recovery?
It is advisable to minimize vibration, balance the centrifuge and use a slightly higher centrifuge speed (2000 g).
Which matrices has IDEXX used in developing Filta-Max xpress system?
IDEXX has experimented with challenging matrices from a wide range of geographical locations.
Is there a chance for cross-contamination when using the Filta-Max xpress Pressure Elution Station?
The elution station uses positive pressure to elute the oocysts and cysts from the filter module, minimizing the risk of contaminating the unit itself. IDEXX supplies five outlet diverters with each elution station so your laboratory can switch diverters between samples. You can clean these manually or in an autoclave. IDEXX has researched the possibility of cross-contamination by using high spikes of target organisms (e.g., 106) and there has not been any evidence of cross-contamination.
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