Enterolert 250

Accurately detects enterococci in 24 hours

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Overview

Easy
  • Ready-to-use media.
  • Simple procedure.
  • Unit-dosed packaging eliminates media preparation.
  • Reagent contains no toxic compounds.
Rapid
  • Confirmed results in 24 hours.
  • Simplified quality control.
  • Less than 1 minute of hands-on time.
Accurate
  • More accurate when compared to ISO 7899-2 on all samples.
  • Liquid enrichment ensures optimized recovery.
  • Eliminates subjective interpretation of results determined by traditional methods.

Science

How the Enterolert 250 Test works

The Enterolert 250 Test uses proprietary Defined Substrate Technology (DST) nutrient indicator to detect enterococci. The Enterolert 250 Test provides confirmed presence/absence results in 24 hours. The Enterolert 250 Test utilizes orth-nitrophenyl-ß-D-glucoside as a nutrient indicator and incorporates a specifically designed blue background color in its formulation.

When the substrate is metabolized by enterococci, the sample turns from blue to green to indicate detection. Any change from the original color to green is considered a positive result. No ultraviolet light source is required. The Enterolert 250 Test detects enterococci at 1 cfu in 250 mL bottled water samples in 24 hours.

How to Use

Learn how to use the Enterolert 250 Test

Enterloert 250
Learn how to use the Enterolert 250 test
 

A two-step guide to using the Enterolert 250 Test

Presence/Absence (P/A)

Step 1

Add reagent to sample, shake to mix and then incubate at 41 ± 1°C for 24 hours; results are valid for up to 28 hours of incubation.

Step 2

Blue indicates negative for enterococci. Green indicates positive for enterococci.

 

Q & A

Frequently asked questions and answers

How does the product work?
The Enterolert 250 Test is designed to detect Enterococcus species in bottled water samples. It is based on IDEXX’s proprietary Defined Substrate Technology (DST). The Enterolert 250 Test utilizes ortho-nitrophenyl-ß-D-glucoside as a nutrient indicator and incorporates a specifically designed blue background color in its formulation. When the enzyme substrate is metabolized by the enterococci, the sample turns from blue to green to indicate a positive detection. Any change from the original color to green is considered a positive result. No ultraviolet light source is required. The Enterolert 250 Test detects enterococci at 1 cfu in 250 mL bottled water samples in 24 hours.

What species of enterococci can the Enterolert 250 Test detect?
The Enterolert 250 Test is designed to detect enterococci in bottled water. As with any microbial method, some Enterococcus species are more easily detected than others. Some examples of Enterococcus species we have detected in trials and studies are:
Enterococcus faecalis
Enterococcus faecium
Enterococcus hirae
Enterococcus gallinarum
Enterococcus casseliflavus
Enterococcus durans
Enterococcus avium

Is there a comparator for the Enterolert 250 Test?
There is no comparator for the Enterolert 250 Test. For comparison, use a negative control when interpreting results.

What is the incubation time and temperature of the Enterolert 250 Test?
The Enterolert 250 Test is incubated at 41°C ± 1.0°C for 24 hours and the results are valid up to 28 hours, unless your local regulations indicate differently.

Are results valid prior to 24 hours?
Yes, if the results are positive. An inoculated Enterolert 250 Test sample that is positive for enterococci before 24 hours is a confirmed positive test for enterococci.

Are results valid after 28 hours?
Yes, if the results are negative. If an inoculated Enterolert 250 Test sample is inadvertently incubated over 28 hours, lack of green color is a valid negative test; a green color after the 28 hours is not valid and should be repeated or verified.

What are the recommended storage conditions for Enterolert 250 Test?
Store the Enterolert 250 Test at 2–25°C, away from direct light and humidity.

What is the normal color change that should take place with the Enterolert 250 Test ?
When the enzyme substrate is metabolized by the enterococci, the sample turns from blue to green to indicate a positive result. Any change from the original color to green is considered a positive result.

What water types can the product be used on/what water type is the product designed to be used for?
The Enterolert 250 Test is designed specifically for bottled water testing.

What color and consistency should the Enterolert 250 reagent powder be?
The Enterolert 250 reagent powder should be white to off-white in color with a slight greenish tint; it should be dry and free-flowing. Any concerns about the color or integrity of the reagent powder should be directed to IDEXX Technical Support.

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Resources & Product Information

Resources

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Product Information

Enterolert 250 Test (20-test pack)
Catalog Number: WENT250-20

IDEXX Water Customer Support

Corporate Headquarters
IDEXX Laboratories, Inc.
One IDEXX Drive
Westbrook, Maine 04092 USA

Tel: 1-800-321-0207
Fax: 1-207-556-4630

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ISO Certifications

File Icon  ISO 9001:2008

File Icon  ISO 17025:2005

File Icon  ISO 14001:2004