IDEXX SNAP® BVDV Antigen Test

For all new introductions to the herd

 

Availability/Distribution: US/Canada Only

The IDEXX SNAP® BVDV Antigen (Ag) Test helps identify bovine viral diarrhea virus-infected cattle in just 20 minutes to prevent the spread of BVDV. By testing all newborn calves, replacements and bulls, veterinarians can help producers ensure the safety of the entire herd and add market value.

 

Sellers of BVDV-tested calves earned, on average, $10 per head more at auction after the cost of testing.1

 

IDEXX recommends a simple three-step management plan (Download the brochure.):

  • Determine your herd status using the IDEXX BVDV PI X2 Test at a local laboratory.
  • Test all new introductions to the herd using the IDEXX SNAP® BVDV Ag Test.
  • Work with your veterinarian to design a vaccination and biosecurity program.

 

Note: IDEXX recommends using the SNAP BVDV Ag Test in conjunction with your local laboratory for high-volume herd surveillance to determine herd status.

 

The IDEXX SNAP BVDV Ag Test in action
See how simple BVDV testing can be with ear-notch sampling. (Download video here.)

 

 

 

Reduce risk and increase ROI with the IDEXX SNAP BVDV Ag Test

Reference

1. Where’s the value? Drovers CattleNetwork. http://www.cattlenetwork.com/drovers/Wheres-thevalue-195684561.html Accessed September 4, 2013

SNAP BVDV Test
 
The IDEXX SNAP BVDV Antigen Test is now available worldwide. Contact an IDEXX representative today to order or to learn more.
Call now to order
United States and Canada
1 800 548 9997
Europe and United Kingdom
00800-727-43399
Australia
1300 4 IDEXX (1300 44 33 99)
Email: aus-lpd@idexx.com
New Zealand
0800 102 084

Fast, easy an accurate testing for the detection of BVDV.

 

Rule out BVDV infection

  • Test all new introductions to the herd—newborn calves, replacements and bulls
  • Identify persistently infected (PI) animals in the herd

 

Easy to run

  • Flexible sample types (ear notch or serum)
  • Run on farm or back at the clinic
  • Simple test protocol utilizes reliable IDEXX SNAP® platform

 

Rapid, results in 20 minutes

  • Diagnosis accurately for same-day treatment recommendation
  • Antigen test uses enzyme-linked immunosorbent assay (ELISA) technology

Dependable results for ear-notch and serum samples

 

The IDEXX SNAP BVDV Antigen Test is a highly sensitive and specific test. Its performance is matched to the widely used IDEXX BVDV PI X2 Test.

 

Sensitivity and Specificity

Using the IDEXX SNAP® BVDV Antigen Test

 

Before You Begin

Allow all components to come to room temperature (18–30°C).

 

Test Procedures

Large Ear-Notch | Serum

  

Large Ear-Notch Test

 

1.
Dispense 1.5 mL of conjugate into a vial containing a single ear notch.
 
2.
Cap vial and swirl gently to soak ear notch.

 

3.
Incubate vial at room temperature (18–30°C) for at least 10 minutes (range: 10-60 minutes).

 

4.
Pour liquid contents of the vial into the sample well of the SNAP® device.

 

5.
When color first appears in the activation circle (may take 15–60 seconds), press firmly to activate. You will hear a distinct “snap.” Note: some liquid may remain in the sample well.

 

6.
Wait 10 minutes, then read results (range: 10–15 minutes).

 

 

 

 

  

Serum Test

 

A heating device is needed (such as a heater block, water bath, etc.) capable of maintaining a temperature of 45 +/- 5°C

 

1.
Dispense 3 drops (150 µL) of serum sample into a sample tube; using a fresh pipette, dispense 6 drops (200 µL) of conjugate into the sample tube.

 

2.
Cap the sample tube and gently invert 3–5 times to mix.

 

3.
Place the sample tube in a heater block and incubate for 10 minutes at 45°C (range: 10–20 minutes).

 

4.
Pour the contents of the sample tube into the sample well of the SNAP device.

 

5.
When color first appears in the activation circle (may take 15–60 seconds), press firmly to activate. You will hear a distinct “snap.” Note: some liquid may remain in the sample well.

 

6.
Wait 10 minutes, then read results (range: 10–15 minutes).

 

 

 

 

 

Interpreting Results

 

Assay is valid if:

  • Positive control spot is blue
  • Negative control spot has no color development

 

Positive
Negative

 

For more information, please review the product insert.

Questions and answers about the IDEXX SNAP® BVDV Antigen Test

 

What is the SNAP® BVDV Test?

An easy-to-use test that detects high levels of bovine viral diarrhea virus (BVDV) antigen in ear-notch and serum samples within 20 minutes. The test is based on the same trusted enzyme-linked immunosorbent assay (ELISA) technology that is used in SNAP® tests for small-animal practices and laboratories.

 

How accurate is the SNAP BVDV Test?

The SNAP BVDV Test is a highly sensitive and specific test. The performance is matched to the widely used IDEXX BVDV Ag Test.

 

What sample types can I use with the SNAP BVDV Test?

The SNAP BVDV Test can be run with small or large ear-notch samples or serum samples. We recommend you choose a large ear-notch sample, as this sample type delivers results in 20 minutes without any interference from maternal antibodies. Refer to the test protocol or product insert for more information.

 

If using the serum protocol, could the SNAP BVDV Test show invalid results due to maternal antibody interference?

Positive results from this assay are valid for calves of any age. Serum should be obtained from precolostral newborn calves or calves older than 3 months of age to prevent the presence of maternal anti-BVDV antibodies from interfering with the SNAP BVDV Test. For more information, please refer to the product insert or contact IDEXX Technical Services.

 

My herd has been vaccinated. Do I still need to test?

Yes, vaccination is not enough to eliminate BVDV. Along with vaccination and biosecurity programs, testing new introductions to the herd can help stop the spread of the disease.

 

What is the likelihood that the SNAP BVDV Test will detect an acute or transient infection (TI)?

It’s highly unlikely that the SNAP BVDV Test would detect anything other than a persistent infection (PI). In most acute cases, the levels of BVDV antigen are expected to be lower and shorter in duration than in persistent infections. However, IDEXX encourages you to confirm a positive result with a second test as outlined in the product insert—especially for high-value animals.

 

The SNAP BVDV Test has been out of the foil pouch for the day. Can I still use it?

We recommend opening the foil pouches immediately before planned usage. However, IDEXX research has found no statistical difference in performance between SNAP® devices handled according to the product insert.

 

Should I be using the SNAP BVDV Test for routine screening of my herds?

Yes, the SNAP BVDV Test should be run on all new introductions to the herd at calving or with replacement heifers. However, IDEXX recommends using your local laboratory for high-volume herd testing.

 

I forgot to refrigerate the SNAP BVDV box when it arrived in the mail. Can I still use the tests?

We recommend refrigerating the box upon arrival at your clinic. Please call IDEXX Technical Services with any specific concerns.

 

Can the SNAP BVDV Test be run in any kind of weather or environment?

The test requires room temperature and a controlled environment. Conditions like excessive sun or rain can affect the accuracy of the result. We suggest running the test inside, for example, the farm office or back at your clinic.

 

Can the SNAP BVDV Test be used with any IDEXX SNAP device reader?

No, at this time the SNAP BVDV Test is not compatible with any IDEXX SNAP Readers. You must read the results visually.

 

Are formalin-fixed tissues OK to use on the SNAP BVDV Test?

No, formalin-fixed tissues cannot be run on the SNAP BVDV test. Formalin will destroy the antigen signal and result in a false-negative test.

 

Are pooled samples OK to run on the SNAP BVDV Test?

No, the SNAP BVDV Test is validated and USDA-approved for individual ear-notch samples and individual serum samples. Pooling samples results in a loss of sensitivity and leads to false- negative results. Further, samples in a pool with neutralizing antibodies could also lead to false- negative results. When testing for BVDV PI animals, it is important not to pool samples on any test.

 
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