Quanti-Disc®
with the Quanti-Disc® Reader

Scientific Basis: How Quanti-Disc Works

Multiple Enzyme Technology™—Quanti-Disc uses IDEXX's Multiple Enzyme Technology (MET™) to detect viable bacteria in water. The reagent contains multiple unique enzyme-substrates, each targeting a different bacterial enzyme. The most common enzymes of waterborne bacteria are all targeted.

All of the enzyme-substrates produce the same signal when metabolized. Therefore, a given waterborne bacteria will be able to metabolize one or more enzyme-substrates and produce a signal. In the case of Quanti-Disc, the signal is fluorescent under 365-nm UV light.

After the sample is added to the center of the disc, the channels and wells fill automatically through capillary action.

After the wells are filled, the disc is incubated with the lid facing up. Since each well is isolated from the others, bacteria from one well will cause only that well to fluoresce.

The number of wells that fluoresce is converted to a Most Probable Number (MPN) using the table provided with the product. The high number of wells (50) results in a high counting range and precise 95% confidence limits. The counting range is from <1.8 to >391 per mL.