Colisure Questions and Answers
Why is the Colisure product irradiated?
We offer low-level gamma-irradiated Colisure product to improve quality and eliminate possible contamination from coliform bacteria. View FAQ's on Irradiation
Is Colisure approved by the U.S. Environmental Protection Agency (EPA)?
Yes. Colisure is US EPA-approved for total coliforms and E. coli in drinking water. Pertinent references:
- U.S. EPA Federal Register, December 5, 1994, Colisure coliform and E. coli approval
- U.S. EPA Federal Register, December 1, 1999, Colisure 24-hour approval
- March 1997, U.S. EPA Manual for the Certification of Laboratories Analyzing Drinking Water, Procedure #5.6
What is the detection limit of Colisure?
Colisure detects coliforms and E. coli at 1 organism/100 mL.
What is the shelf life of Colisure?
Colisure shelf life is up to 12 months from date of manufacture.
What are the recommended storage conditions for Colisure?
2-25°C away from light.
Is there a comparator for Colisure?
No.
Why is the test limited to a 24- to 48-hour incubation?
The reagent system was designed to suppress non-coliform CPRG or MUG positive bacteria for at least 48 hours. If incubation extends past 48 hours, it is possible that such bacteria could overcome the reagent and react with CPRG or MUG. When incubation exceeds 48 hours, only negative results are valid. Positive results must be confirmed.
May samples be read after 48 hours?
If an inoculated Colisure sample is inadvertently incubated over 48 hours, lack of red to magenta color is a valid negative test; a red to magenta color after 48 hours is not valid and should be repeated or confirmed by a traditional method.
May samples be read before 24 hours?
An inoculated Colisure sample that is positive for coliforms and E. coli before 24 hours is a valid positive test for both coliforms and E. coli. If the sample is positive before 24 hours for coliforms only, the sample is a valid positive test for total coliforms. However, the sample should be incubated for a longer period (at least 24 hours, but not greater than 48 hours total time) to determine whether E. coli are present.
Can I use Colisure with Quanti-Tray® and Quanti-Tray®/2000?
Yes. When using Colisure with Quanti-Tray, Quanti-Tray/2000 or any multiple tube format, be sure to use an IDEXX vessel with antifoam or IDEXX Antifoam Solution.
What are the disposal requirements for a Colisure sample?
Check with your local, state and/or federal authorities for proper disposal of bacteriological biohazard materials at your facility.
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