Colisure®Coliform/E. coli Results in 24 Hours Colisure coliform/E.coli results are definitive at 24 hours and can be read for up to 48 hours for convenience. This test's distinct magenta color means there's no mistaking a positive result. Colisure also suppresses high levels of non-coliforms including aeromonas.
Colisure is US EPA-approved and is included in Standard Methods for Examination of Water and Wastewater.
Colisure® has a read window that lasts between 24 and 48 hours |
Colisure® is easy to use, rapid and accurate
Easy
- Definitive results at 24 hours, can be read up to 48 hours.
- Distinct magenta endpoint is easy to see.
- Ease of use simplifies training.
- Unit-dosed packaging eliminates media preparation.
- Quality Control (QC) procedure can be done in 15 minutes.
Rapid
- Under one minute hands-on time.
- Detects total coliforms and E. coli simultaneously in 24 hours or less.
- No confirmations needed.
- No glassware cleaning or colony counting.
Accurate
- Identifies E. coli specifically, which eliminates unneeded public notification due to Klebsiella pneumoniae.
- Suppresses up to 2 million heterotrophs per 100 mL.
- Eliminates the subjective interpretation found in traditional methods.
- Detects a single viable coliform or E. coli per sample.
Economical
- 95% less equipment costs than membrane filtration (MF).
- Minimizes evening and weekend work.
- Up to 12-month shelf life at room temperature.
Flexible
- A Colisure® Snap Pack can be used for presence/absence (P/A) or quantification testing.
- Quanti-Tray® provides counts to 200/100 mL without dilutions.
- Quanti-Tray®/2000 provides counts to 2,419/100 mL without dilutions.
Scientific Basis: How Colisure® Works
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Colisure® uses Defined Substrate Technology® (DST®) nutrient indicators CPRG and MUG to detect total coliforms and E.coli. Coliforms use their β-galactosidase enzyme to metabolize CPRG and change it from yellow to magenta. E.coli use β-glucuronidase to metabolize MUG and create fluorescence.
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Colisure Questions and Answers
Why is the Colisure product irradiated? We offer low-level gamma-irradiated Colisure product to improve quality and eliminate possible contamination from coliform bacteria. View FAQs on irradiation. Is Colisure approved by the U.S. EPA? Yes, Colisure is U.S. Environmental Protection Agency (EPA)-approved as a method for total coliform and E. coli detection in drinking water and for E. coli detection in the following water:
What is the detection limit of Colisure? Colisure detects coliforms and E. coli at 1 organism/100 mL. What is the shelf life of Colisure? Colisure shelf life is up to 12 months from date of manufacture. Why is the test limited to a 24- 48-hour incubation? The reagent system was designed to suppress non-coliform CPRG or MUG positive bacteria for at least 48 hours. If incubation extends past 48 hours, it is possible that such bacteria could overcome the reagent and react with CPRG or MUG. When incubation exceeds 48 hours, only negative results are valid. Positive results must be confirmed. Can samples be read after 48 hours? If an inoculated Colisure sample is inadvertently incubated over 48 hours, lack of red to magenta color is a valid negative test; a red to magenta color after 48 hours is not valid and should be repeated or confirmed by a traditional method. Can samples be read before 24 hours? An inoculated Colisure sample that is positive for coliforms and E. coli before 24 hours is a valid positive test for both coliforms and E. coli. If the sample is positive before 24 hours for coliforms only, the sample is a valid positive test for total coliforms. However, the sample should be incubated for a longer period (at least 24 hours, but not greater than 48 hours total time) to determine whether E. coli are present. Can I use Colisure with Quanti-Tray® and Quanti-Tray®/2000? Yes, when using Colisure with Quanti-Tray, Quanti-Tray/2000 or any multiple tube format, be sure to use an IDEXX vessel with antifoam or IDEXX Antifoam Solution. What are the disposal requirements for a Colisure sample? Check with your local, state and/or federal authorities for proper disposal of bacteriological biohazard materials at your facility. |
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