SNAP® Giardia Test

For dogs and cats—every fecal workup deserves a SNAP® Giardia Test

 

The SNAP Giardia Test:

  • Detects soluble Giardia antigens—no more slide-scanning for evasive cysts
  • Has no sample-prep time—easy to set up alongside fecal floats
  • Provides better answers on just one sample—near reference-laboratory accuracy
  • Is the first USDA-approved in-clinic rapid assay for the detection of Giardia-solution antigen
SNAP Giardia Test

Here’s how a comprehensive fecal-testing protocol can help your practice

 

  • Reliable and cost-effective, the SNAP®Giardia Test dramatically improves fecal examination accuracy by including a fecal float and a 90% sensitive Giardia-only test.
  • Catch missed Giardia-positive animals up to three times as often as traditional methods1—increasing your fecal exam yield and client satisfaction
  • Send more patients home with a definitive diagnosis and targeted treatment plan
  • Improve your fecal exam revenues

 

We use the SNAP Giardia Test as part of our baseline fecal screen for all young animals and all clinical GI workups because we have found Giardia to be prevalent, and it helps us provide the best care for our patients. Additionally, it’s very easy to use, convenient for our clients and hospital, and is a good profit source with very high perceived value to our clients as we find, treat and clear more positives. As I tell my colleagues, ‘mandate and uphold the best protocol.’ This isn’t just good business, it’s good medicine.

Joseph R. Sprott, DVM • Newnan Animal Hospital • Newnan, Georgia

 

Use the SNAP Giardia Test as an adjunct to fecal float parasitic diagnosis—if you’re looking for intestinal parasites, don’t miss a Giardia infection.

The Companion Animal Parasite Council (CAPC) “recommends testing symptomatic (intermittently or consistently diarrheic) dogs and cats with a combination of direct smear, fecal flotation with centrifugation, and a sensitive, specific fecal ELISA optimized for use in companion animals.”2

Misdiagnosis results from intermittent shedding and difficulty identifying cysts and trophozoites. At present, the SNAP Giardia Test is the only commercially available Giardia ELISA assay optimized for use in canine and feline samples.

The IDEXX Test Promise —If an IDEXX test does not perform as promised, simply call us and we will credit your IDEXX Points account with 100% of the test’s value in points. If you have questions about the IDEXX Test Promise, please call us at 1–800–248–2483.

 

References
1.
Groat R. Survey of clinic practices and testing for diagnosis of Giardia infections in dogs and cats. Presented at: 2003 ACVIM Forum; June 4–8; Charlotte, NC.
2.
CAPC. CAPC Recommendations: Intestinal Parasites: Protozoa: Giardiasis. Available at: www.capcvet.org/recommendations/giardia.html. Accessed May 27, 2009.

Sensitivity and specificity of the SNAP® Giardia Test

SNAP Giardia test sensitivity and specificity

CL = confidence limit

 

Compare Giardia testing methods to see the difference.
 Light MicroscopyLaboratory IFALaboratory ELISA
SNAP Giardia
Identifies soluble Ag  
Time to result~20 mins24–48 hours24–48 hours8 minutes
Inexpensive  
In-house  
Samples required3111
High accuracy 

CONCLUSION: SNAP®Giardia—the ease of fecal floats/smears with the accuracy of a reference laboratory.

 

Detecting Giardia Infection: A Comparison of In-House Methods vs. Reference Laboratory Methods

SNAP Giardia compared to other testing methodsSNAP Giardia compared to other testing methods

SNAP®Giardia Test and in-clinic microscopy test results are shown as percentages of samples that were identified as Giardia-positive by laboratory ELISA and DIFM methods.

 

In a recent study, 617 fecal samples were tested for Giardia in the normal course of clinical practice.1 Results from fecal floatation or fecal smears were recorded, and the samples were then forwarded for further reference testing. The results indicated poor performance of current in-house microscopy testing compared to ELISA and DIFM reference methods.

SNAP®Giardia Test and in-clinic microscopy test results are shown as percentages of samples that were identified as Giardia-positive by laboratory ELISA and DIFM methods.

 

 

Reference
1.
Groat R. Survey of clinic practices and testing for diagnosis of Giardia infections in dogs and cats. Presented at: 2003 ACVIM Forum; June 4–8; Charlotte, NC.

Resources and support materials for the SNAP® Giardia Test

It’s easy to use the SNAP® Giardia Test:
1.
Swab sample and place swab tip into tube. Bend bulb to break seal and release conjugate.
2.
Squeeze and release bulb 3 times to mix sample and conjugate.
3.
Squeeze bulb to dispense 5 drops into the sample well of a SNAP® device.
4.
When color first appears in the activation circle, press firmly to activate. You will hear a distinct “snap.”
5.
Read the test result
8 minutes from the time of activation.

View the package insert (PDF) for detailed instructions.

Interpreting Results

To determine the test result, read the reaction spots in the result window and compare the color intensity of the sample spot to that of the negative control spot.

Negative Result
The result is negative for a sample spot, if:

  • There is no color on the sample spot and the negative control spot.
    or
  • Color on the sample spot is equal to the color on the negative control spot.

Positive Result
Color on the Giardia Ag indicator sample spot is darker than the color on the negative control spot. Note: Some positive results may have only light color intensity on the sample spot.

Invalid Result
The negative control spot serves as a safeguard against false-positives. Color development on the positive control spot indicates the test reagents are functional and helps indicate that the assay has been run properly.

  • If the positive control spot does not develop color, the result is invalid. Repeat the test.
  • If color on the negative control spot is darker than color on the sample spot, the test is invalid. Repeat the test.
  • If invalid results are obtained for a sample when repeated, contact IDEXX Customer Support.

Questions and answers about the SNAP® Giardia Test

 

How does the SNAP Giardia Test’s sensitivity and specificity compare to other techniques?

Groat R. Survey of clinic practice and testing for diagnosis of Giardia infections in dogs and cats. Presented at: 2003 ACVIM Forum; June 4–8; Charlotte, NC. SNAP® Giardia Test and in-clinic microscopy test results are shown as percentages of samples that were identified as Giardia-positive by laboratory ELISA and DIFM methods. The SNAP Giardia Test provides near-reference laboratory accuracy and is far superior to the poor performance of traditional in-clinic methods.

 

Do you have any information/data on the comparison of the SNAP Giardia Test to other methods?

 Light MicroscopyLaboratory IFALaboratory ELISA
SNAP Giardia
Identifies soluble Ag  
Time to result~20 mins24–48 hours24–48 hours8 minutes
Inexpensive  
In-house  
Samples required3111
High accuracy 
CONCLUSION: SNAP® Giardia—the ease of fecal floats/smears with the accuracy of a reference laboratory.

 

What type of Giardia does your test detect?

It identifies Giardia lamblia, otherwise known as Giardia intestinalis or Giardia duodenalis, the primary species in mammals.

 

What is soluble antigen?

This refers to antigen that is not associated with a Giardia cyst wall or trophozoite. Our test detects a “free-floating” antigen that is dispersed in the fecal matter. This is why our sensitivity is much higher that microscopic techniques. (See ELISA Technology)

 

How should a sample be saved?

Canine and feline feces can be used fresh, previously frozen or stored at 2°–7°C (36°–45°F) for up to seven days.

 

Can this test be used for avian samples?

The SNAP Giardia Test is approved for canine and feline use only. Any use other than in canine and feline is off-label, and IDEXX has no data to support non-canine/feline application.

 

How should the SNAP Giardia Test Kit be stored?

SNAP devices and test reagents are stable until the expiration date when stored at 2°–7°C (36°–45°F). Please see the package insert (PDF) for additional information.

 

My SNAP Giardia Test has been out of the foil package for the day. Can I still use it?

The SNAP Giardia Test, and any other SNAP® test, must be used within two hours of removing it from the foil package.

 
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