SNAP® BVD Test

For all new introductions to the herd

Availability/Distribution: Worldwide

The SNAP® BVD Test helps identify BVD-infected cattle in just 20 minutes to prevent the spread of BVDV. Test all newborn calves, replacements and bulls to ensure the safety of the entire herd.

U.S. veterinarians estimate that BVDV costs beef and dairy producers over $30 a head after vaccination.1 Now it doesn’t have to.

By testing all new introductions to the herd, veterinarians can help producers avoid the costly impact of BVDV. IDEXX recommends a simple three-step management plan:

  • Determine herd status; PI cattle are costly and present at all stages of production in dairy and beef herds.
  • Test all new introductions with the SNAP BVD Test.
  • Work with your veterinarian to design a vaccination and biosecurity program.

 

Note: IDEXX recommends using the SNAP BVD Test in conjunction with your local laboratory for high-volume herd surveillance to determine herd status.

The SNAP BVD Test in action
See how simple BVDV testing can be with ear-notch sampling. (Download video here.)

SNAP BVD Test in Action

 

 

Reference

1. Data on file at IDEXX Laboratories, Inc. Westbrook, Maine USA.

SNAP BVD Test
The SNAP BVD Test is now available worldwide. Contact an IDEXX representative today to order or to learn more.
Call now to order
United States and Canada
1 800 548 9997
Europe and United Kingdom
00800-727-43399
Australia
1300 4 IDEXX (1300 44 33 99)
Email: aus-lpd@idexx.com
New Zealand
0800 102 084

Fast, easy an accurate testing for the detection of BVDV.

Rule out BVDV infection

  • Test all new introductions to the herd—newborn calves, replacements and bulls
  • Identify persistently infected (PI) animals in the herd

Easy to run

  • Flexible sample types (ear notch or serum)
  • Run on farm or back at the clinic
  • Simple test protocol utilizes reliable IDEXX SNAP® platform

Rapid, results in 20 minutes

  • Diagnosis accurately for same-day treatment recommendation
  • Antigen test uses enzyme-linked immunosorbent assay (ELISA) technology

Dependable results for ear-notch and serum samples

The SNAP BVD Test is a highly sensitive and specific test. Its performance is matched to the widely used IDEXX BVDV Ag Test.

 

Sensitivity and Specificity

Using the SNAP® BVD Test

 

Before You Begin

Allow all components to come to room temperature (18–30°C).

 

Test Procedures

Large Ear-Notch | Serum

  

Large Ear-Notch Test

 

1.
Dispense 1.5 mL of conjugate into a vial containing a single ear notch.
 
2.
Cap vial and swirl gently to soak ear notch.

 

3.
Incubate vial at room temperature (18–30°C) for at least 10 minutes (range: 10-60 minutes).

 

4.
Pour liquid contents of the vial into the sample well of the SNAP® device.

 

5.
When color first appears in the activation circle (may take 15–60 seconds), press firmly to activate. You will hear a distinct “snap.” Note: some liquid may remain in the sample well.

 

6.
Wait 10 minutes, then read results (range: 10–15 minutes).

 

 

 

 

  

Serum Test

 

Heater block (available through IDEXX) required for use with serum sample. Please contact your IDEXX representative for more information.

 

1.
Dispense 3 drops (150 µL) of serum sample into a sample tube; using a fresh pipette, dispense 6 drops (200 µL) of conjugate into the sample tube.

 

2.
Cap the sample tube and gently invert 3–5 times to mix.

 

3.
Place the sample tube in a heater block and incubate for 10 minutes at 45°C (range: 10–20 minutes).

 

4.
Pour the contents of the sample tube into the sample well of the SNAP device.

 

5.
When color first appears in the activation circle (may take 15–60 seconds), press firmly to activate. You will hear a distinct “snap.” Note: some liquid may remain in the sample well.

 

6.
Wait 10 minutes, then read results (range: 10–15 minutes).

 

 

 

 

 

Interpreting Results

 

Assay is valid if:

  • Positive control spot is blue
  • Negative control spot has no color development

 

Positive
Negative

 

For more information, please review the product insert.

Frequently asked questions about the SNAP® BVD Test

 

What is the SNAP® BVD Test?

An easy-to-use test that detects high levels of bovine viral diarrhea virus (BVDV) antigen in ear-notch and serum samples within 20 minutes. The test is based on the same trusted enzyme-linked immunosorbent assay (ELISA) technology that is used in SNAP® tests for small-animal practices and laboratories.

 

How accurate is the SNAP BVD Test?

The SNAP BVD Test is a highly sensitive and specific test. The performance is matched to the widely used IDEXX BVDV Ag Test.

 

What sample types can I use with the SNAP BVD Test?

The SNAP BVD Test can be run with small or large ear-notch samples or serum samples. We recommend you choose a large ear-notch sample, as this sample type delivers results in 20 minutes without any interference from maternal antibodies. Refer to the test protocol or product insert for more information.

 

If using the serum protocol, could the SNAP BVD Test show invalid results due to maternal antibody interference?

Positive results from this assay are valid for calves of any age. Serum should be obtained from precolostral newborn calves or calves older than 3 months of age to prevent the presence of maternal anti-BVDV antibodies from interfering with the SNAP BVD Test. For more information, please refer to the product insert or contact IDEXX Technical Services.

 

My herd has been vaccinated. Do I still need to test?

Yes, vaccination is not enough to eliminate BVDV. Along with vaccination and biosecurity programs, testing new introductions to the herd can help stop the spread of the disease.

 

What is the likelihood that the SNAP BVD Test will detect an acute or transient infection (TI)?

It’s highly unlikely that the SNAP BVD Test would detect anything other than a persistent infection (PI). In most acute cases, the levels of BVDV antigen are expected to be lower and shorter in duration than in persistent infections. However, IDEXX encourages you to confirm a positive result with a second test as outlined in the product insert—especially for high-value animals.

 

The SNAP BVD Test has been out of the foil pouch for the day. Can I still use it?

We recommend opening the foil pouches immediately before planned usage. However, IDEXX research has found no statistical difference in performance between SNAP® devices handled according to the product insert.

 

Should I be using the SNAP BVD Test for routine screening of my herds?

Yes, the SNAP BVD Test should be run on all new introductions to the herd at calving or with replacement heifers. However, IDEXX recommends using your local laboratory for high-volume herd testing.

 

I forgot to refrigerate the SNAP BVD box when it arrived in the mail. Can I still use the tests?

We recommend refrigerating the box upon arrival at your clinic. Please call IDEXX Technical Services with any specific concerns.

 

Can the SNAP BVD Test be run in any kind of weather or environment?

The test requires room temperature and a controlled environment. Conditions like excessive sun or rain can affect the accuracy of the result. We suggest running the test inside, for example, the farm office or back at your clinic.

 

Can the SNAP BVD Test be used with any IDEXX SNAP device reader?

No, at this time the SNAP BVD Test is not compatible with any IDEXX SNAP Readers. You must read the results visually.

 

Are formalin-fixed tissues OK to use on the SNAP BVD Test?

No, formalin-fixed tissues cannot be run on the SNAP BVD test. Formalin will destroy the antigen signal and result in a false-negative test.

 

Are pooled samples OK to run on the SNAP BVD Test?

No, the SNAP BVD Test is validated and USDA-approved for individual ear-notch samples and individual serum samples. Pooling samples results in a loss of sensitivity and leads to false- negative results. Further, samples in a pool with neutralizing antibodies could also lead to false- negative results. When testing for BVDV PI animals, it is important not to pool samples on any test.

 
Related Products