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B. abortus
Brucella abortus
Brucellosis in cattle is a disease caused by Brucella abortus (B. abortus), a facultative, intracellular bacterium. The major
mode of disease transmission is ingestion of B. abortus organisms that may be present in tissues of aborted fetuses,
fetal membranes and uterine fluids. In addition, infection may occur as the result of cattle ingesting B. abortus-contaminated
feed or water. Infection in cows has also occurred through venereal transmission of the organism by
infected bulls.
Abortion is the outstanding clinical feature of the disease. If the carrier state develops in a majority of infected cows
in a herd, the clinical manifestations may be reduced milk production, dead calves at term and a higher frequency of
retained placentas. Disease in the bull may produce infections of the seminal vesicles and testicles, resulting in shedding of
the organisms in semen.
Diagnosis is based on serological (serum/milk) and bacteriological procedures. While a positive bacteriological
finding is the most definitive diagnosis, several weeks may be required to obtain final culture results. The success of
disease eradication depends upon rapid and accurate identification.
Testing Solutions
CHEKIT* Brucella abortus Antibody Test Kits
 Availability/Distribution: Outside the U.S.
The CHEKIT-Brucellose-Serum ELISA Test Kit is an enzyme immunoassay for the detection of antibodies against Brucella abortus in individual serum and plasma, and pools of up to 10 individual serum or plasma samples of ruminants.
The CHEKIT-Brucellose-Milk ELISA Test Kit is an enzyme immunoassay for the detection of antibodies against Brucella abortus in individual or pooled bovine milk of up to 250 cows.
The CHEKIT B. abortus Test Kit includes chemical reagents (wash, substrate, stop solutions) that can also be used with
CHEKIT kits that test for other abortive diseases (Q fever, neosporosis, toxoplasmosis, Chlamydia).
CHEKIT* Brucellose Serum Antibody ELISA:
10 plates (strips), serum and plasma samples (individual or pool up to 10), short and overnight protocols (bovine, ovine)
CHEKIT* Brucellose Milk Antibody ELISA:
10 plates (solid), milk samples (individual or pool up to 250), short and overnight protocols (bovine)
HerdChek* Brucella abortus Antibody Test Kit
 Availability/Distribution: U.S. only
HerdChek* B. abortus Antibody PCFIA:
10,560 tests per kit, serum samples, recognized as an official test in the U.S. (bovine)
* xChek, CHEKIT, FlockChek and HerdChek are trademarks or registered trademarks of IDEXX Laboratories, Inc. in the United States and/or other countries. Pourquier is a trademark of Institut Pourquier, a wholly owned subsidiary of IDEXX Laboratories, Inc.
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